Fascination About attune flow cytometer
Fascination About attune flow cytometer
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At that time, the early indications had been dismissed as debris, however the retrospective analysis shown shared characteristics Along with the problematic cells in lifestyle. Tracing the an infection aided the lab set up extra laboratory techniques for screening and defense of assay-important mobile traces.
As samples are acquired about the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digital camera captures and shops pictures of detected occasions at a fee nearly six,000 visuals for every next, depending on the flow charge and image size. For bigger adaptability, Attune Cytometric Computer software lets you alter the picture seize frequency as needed and choose a certain gate for impression seize, if expected.
Merge acoustic concentrating technology with significant-parameter Assessment for flexible spectral unmixing and standard workflows.
Find out new insights into your experimental data by visualizing populations making use of combined fluorescence and morphology facts. Our software program interface wizards permit downsampling, concatenation (merged information) and dimensionality tools which have been created into your application for an intuitive, streamlined data Evaluation working experience.
"An image is just not fairly worthy of 1000 dots, but it truly really helps to see photographs. It is great to check out photos that correlate with staining. Such as, AnnexinV-beneficial cells that happen to be morphologically unique than healthier cells, or CD14+ monocytes which can be bigger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it is actually illuminating to determine how many doublets slip via classic singlet gates, and fantastic to verify the standing of sudden double-positive situations in my Examination."
Pair your Attune flow cytometer with the autosampler for greater workflow effectiveness, and increase robotic integration for maximum throughput.
Dependable picture high-quality even at higher throughput Acoustic focusing in addition to a substantial-speed camera Blend to picture these T cells consistently at minimal or significant flow charges. Conveniently regulate focus and digicam options to meet experimental needs.
Early signs of this contamination experienced in the beginning been dismissed as particles. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also enables you to use morphological options to find out attention-grabbing subpopulations that may not be clear from flow cytometry knowledge by yourself.
Perhaps most of all, these gatherings comprise cells of Plainly distinct phenotypes which could lead to incorrect conclusions relating to double positive events (particularly in exceptional populations).
Sound waves enable exact cell alignment, leading to exceptional information high quality and ~10X more rapidly sample flow premiums.
Software Be aware: Automated graphic Examination lessens person-to-consumer variability in flow cytometry gating procedures
Created-in dimensionality reduction equipment improved resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate anticipated populace resolution in lysed whole human blood. User can look at occasions from Each and every cluster for visual affirmation of cell phenotypes.
No clean/no lyse leukocyte Assessment with imaging. Cells were obtained from 24-hour-aged blood by dilution in one mM EDTA ( Expert UX/UI enables end users to teach AI products for expanded purposes. (A) The “Prepare Product” element offers annotation applications and actual-time product check here schooling precision.
At the guts of every Attune Flow Cytometer lies our innovative acoustic concentrating know-how, which makes use of audio waves to meticulously align cells within the flow mobile. This ground breaking method surpasses classic hydrodynamic techniques, leading to more quickly processing speeds, Extraordinary facts high quality, and elevated sample throughput.
Since the cell line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went again to the pictures and had been in a position to doc the microbial infection a minimum of 5 times earlier.
Agilent Value Guarantee ensures you a minimum of ten years of instrument use from your date you buy. If not, we will credit rating you with the residual price of the procedure toward an upgraded product.
What gave the impression to be a homogeneous populace is really far more numerous—an insight that needs to be considered when interpreting results.